Dekokta Scurulla atropurpurea Terhadap Kelengkungan Tulang Belakang Embrio Ikan Zebra
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Jun 30, 2018
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Abstract
Toxicity study on herb Scurulla atropurpurea has done by using Zebra fish (Danio rerio) embryo. The objective of this research is to study the effect of decocta of parasitic tea leaves on theraphy dosage, MATC, and LC50 to the backbones of Zebra fish embryo. This research is done by laboratory experiment method through in vitro by using research design post randomized control group only. The animal used in this research is Zebra fish embryo less than 3 hours post fertilization after fission process is finished and embryo is entering blastula stage. Total number of sample is 80 embryo and divided into 4 groups: control group without treatment, therapy dosage group (200ug/ml), LC50 group (2066ug/ml) and MATC group (237ug/ml) by decocta of parasitic tea leaves. Sample of Zebra fish embryo is putting in well-plate with 1 embryo per well. Treatment was given untill embryo at 72hpf at temperature 270 C. Data taken during study is analysed using one way ANOVA by SPSS version 16. Treatment with therapy dosage 200, MATC 237 and LC50 of decocta of parasitic tea leaves do not cause curvature of backbone in Zebra fish embrio. It is suspected that active compound in decocta of parasitic tea leaves at all above dosage do not able to disturb the structure and function of backbone.
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References
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Athiroh, N. (2009b). Pemaparan Scurulla oortiana (benalu teh) dan Macrosolen javanus (benalu jambu mawar) setelah stimulasi listrik terhadap kontraksi pembuluh darah arteri ekor tikus. Prosiding Bioteknologi. Seminar Nasional Biologi XX dan Kongres PBI XIV. Malang: 58-63. ISBN 978-602-95471-0-8
Athiroh, N. (2010). Effect of Scurulla oortiana and Macrosolen Javanus for vasodilator of blood vessel. Prosiding. SEMNAS Basic Science VII UB. Malang : 271-277. ISBN: 978-602-96393-0-8.
Athiroh, N and N. Permatasari. (2012). Mechanism of tea mistletoe action on blood vessels. Medical Journal Brawijaya, 27 (1), 1-4.
Athiroh, N and E. Sulistyowati. (2013). Scurrula atropurpurea increases Nitric Oxide and decreases malondialdehyde in hypertensive rats. Univ Med, 32, 44-50.
Athiroh N., N. Permatasari, D. Sargowo, & M. A. Widodo. (2014a). Antioxidative and blood pressure-lowering effects from Scurrula atropurpurea on DOCA-salt hypertensive rats. Biomarkers and Genomic Medicine Journal, 1, 32-36.
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Athiroh, N., E. Sulistyowati. (2015). Extract of Scurrula atropurpurea (Bl.) Dans sub chronic exposure on wistar rat liver. AENSI Journals Advances in Environmental Biology, 9(23), 245-250.
Athiroh, N. & Wahyuningsih, D. (2017). Study of superoxide dismutase and malondialdehyde concentrations in mice after administration of methanolic extract of Scurrula atropurpurea (Bl.). Jurnal Kedokteran Hewan, 11 (1). 19-22.
Athiroh, N. (2017). Monograf. Benalu teh dan hipertensi. Intelligensia Media. Malang Indonesia.
Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 7 Tahun (2014) Tentang Pedoman Uji Toksisitas Nonklinik Secara In Vivo.
de Moura, R.S., A.F. Emiliano, L.C.R. Marins de Carvalho, L.C.R. Souza, D.C. Guedes, T. Tano, A.C. Resende. (2005). Antihypertensive and endothelium-dependent vasodilator effects of Alpinia zerumbet, a medicinal plant. J Car diovasc PharmacolTM, 46, 288–294.
Detrich, H.W., Westerfield, M., Zon, L.I. (2009). Essential zebrafish method : Genetics and genomics. Elseivier. 1st edition. U.K
Eno, A.E., U.E. Ibokette, Ofem, F.B. Uoh, E. Nkanu, N. Azah, J.O. Ibu. (2004). The effects of a negerian species of Viscum album (mistletoe) leaf extraction on the blood pressure of normotensive and DOCA_induced hypertensive rats. Nigerian Journal of Physiological Sci, 19 (1-2), 33-38.
Halliwell, B. and M.C. Gutteridge. (2010). Role of free radicals and catalytic metal ions in human disease. Oxford. Clarendon Press.
Hanien, D.N., Firmansyah, M., Athiroh, N. (2016). Nilai LC50 ikan zebra dewasa yang dipapar oleh dekokta daun benalu teh (Scurulla atropurpurea Bl.Danser) secara akut dan subkronik. Jurnal Bio Komplementer Medicine, 3 (1), 65-70.
Hasri, Y.U., Firmansyah, M., Athiroh, N. (2016). Nilai LCC0 embrio dan larva ikan zebra (Danio rerio) yang dipapar dekokta daun benalu teh (Scurrula atropurpurea Bl. dans.) secara akut. Jurnal Bio Komplementer Medicine, 3 (1), 59-64.
Hikmah, U., Athiroh,, N., Santoso, H. (2017). Kajian sub kronik ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap serum glutamic oxaloacetic transaminase tikus wistar betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2 (2), 30-35.
Jiménez, R., R. López-Sepúlveda , M. Kadmiri , M. Romero , R. Vera , M. Sánchez , F. Vargas , F. O'Valle , A. Zarzuelo , M. Dueñas , C. Santos-Buelga , J. Duarte. (2007). Polyphenols restore endothelial function in DOCA-salt hypertension: role of endothelin-1 and NADPH oxidase. Free Radic Biol Me,. 43(3), 462-73.
Kawamura, E., & Pamudji. (2005). Pharmaceutical composition containing powder or extract of a paraite plant of the loranthaceae family. European Patent Aplication. EP 1 591 126 A2.
Langgheinrich, U. (2003). Bioassay. Vol. 25. 904-912.
Leischke, G.J., & Currie, P.D. (2007). Animal models for human desease: Zebrafish swim into view. Nat Pub Group. 8 : 353-367.
Mahyan, A., Athiroh, N., Santoso, H. (2016). Paparan 28 hari ekstrak metanolik Scurrula atropurpurea terhadap kadar SGPT tikus betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(1), 53-58.
Munawaroh, N.S., Athiroh, N., Santoso, H. (2016). Kajian ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap kadar trigliserida pada tikus wistar betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(1), 59-64.
Murphy, KJ., A.K. Chronopolous, I. Singh, M.A. Francis, H. Moriarty, M.J. Pike, A.H. Turner, N.J. Mann, and J. Sinclair. (2003). Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function. American Journal of Clinical Nutrition, 77 (6), 1466-1473.
Mustofa, B., A. Hayati, dan N. Athiroh. (2013). Peran ekstrak metanolik benalu teh terhadap perbaikan stress oksidatif melalui peningkatan SOD pada tikus hipertensi. Jurnal Ilmiah Bio saintropis, 1 (2), 1-4.
Nugroho, R.T., Herbani, M., Athiroh, N. (2016). Efek dekokta daun benalu teh (Scurrula atropurpurea BL Danser) dosis terapi, MATC, dan LC50 terhadap denyut jantung dan daya tetas embrio ikan zebra (Danio rerio). Jurnal Bio Komplementer Medicine, 3 (1), 51-58.
Ortiz, P.A., and J.L. Garvin. (2001). Intrarenal transport and vasoactive substances in hypertension. Hypertension, 38, 621–624.
Purnamasari, D., Herbani, M., Athiroh, N. (2016). Efek dekokta daun benalu teh (Scurrula atropurpurea Bl. Dans) dosis terapi, MATC, dan Lc50 terhadap luas area yolk sac serta pericardium embrio ikan zebra (Danio rerio). Jurnal Bio Komplementer Medicine, 3 (1), 167-173.
Prastika, N.I., Athiroh, N., Santoso, H. (2017). Pengaruh pemberian sub kronik ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap kadat kreatinin tikus wistar. e-Jurnal Ilmiah BIOSAINTROPIS, 2(2), 42-48.
Redon, J.M., M.R. Oliva, C. Tormos, V. Giner, J. Chaves, A. Iradi, G.T. Saez. (2003). Antioxidant activities and oxidative sress byproducts in human hypertension. Journal of The American Heart Association, 41, 1096-1101.
Sammad, F.A.H., Athiroh, N., Santoso, H. (2017). Pemberian ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. secara sub kronik terhadap protein total dan albumin tikus betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(2), 49-54.
Vaziri, N.D., and B. Rodriguez-Iturbe. 2006. Mechanisms of Disease: Oxidative Stress and Inflammation in The Pathogenesis of Hypertension. Nat Clin Pract Nephrol, 282–593.
Wang, S., Kechun Liu, Ximing Wang, Qiuxia He, and Xiqiang Chen. (2010). Toxic effect of celastrol on embryonic development of zebra fish (Danio rerio). Biology Institute of Shandong Academy of Sciences, Jinan 250014, Shandong, China.
Athiroh, N. (2009a). Kontraktilitas pembuluh darah arteri ekor tikus terpisah dengan atau tanpa endotel setelah pemberian ekstrak Scurulla oortiana (benalu teh). Jurnal Berkala Hayati. No.3D. ISSN: 0852-6834.
Athiroh, N. (2009b). Pemaparan Scurulla oortiana (benalu teh) dan Macrosolen javanus (benalu jambu mawar) setelah stimulasi listrik terhadap kontraksi pembuluh darah arteri ekor tikus. Prosiding Bioteknologi. Seminar Nasional Biologi XX dan Kongres PBI XIV. Malang: 58-63. ISBN 978-602-95471-0-8
Athiroh, N. (2010). Effect of Scurulla oortiana and Macrosolen Javanus for vasodilator of blood vessel. Prosiding. SEMNAS Basic Science VII UB. Malang : 271-277. ISBN: 978-602-96393-0-8.
Athiroh, N and N. Permatasari. (2012). Mechanism of tea mistletoe action on blood vessels. Medical Journal Brawijaya, 27 (1), 1-4.
Athiroh, N and E. Sulistyowati. (2013). Scurrula atropurpurea increases Nitric Oxide and decreases malondialdehyde in hypertensive rats. Univ Med, 32, 44-50.
Athiroh N., N. Permatasari, D. Sargowo, & M. A. Widodo. (2014a). Antioxidative and blood pressure-lowering effects from Scurrula atropurpurea on DOCA-salt hypertensive rats. Biomarkers and Genomic Medicine Journal, 1, 32-36.
Athiroh N., N. Permatasari, D. Sargowo, and M.A. Widodo. (2014b). Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats. Iran J Basic Med Sci, 17, 622-625.
Athiroh, N., E. Sulistyowati. (2015). Extract of Scurrula atropurpurea (Bl.) Dans sub chronic exposure on wistar rat liver. AENSI Journals Advances in Environmental Biology, 9(23), 245-250.
Athiroh, N. & Wahyuningsih, D. (2017). Study of superoxide dismutase and malondialdehyde concentrations in mice after administration of methanolic extract of Scurrula atropurpurea (Bl.). Jurnal Kedokteran Hewan, 11 (1). 19-22.
Athiroh, N. (2017). Monograf. Benalu teh dan hipertensi. Intelligensia Media. Malang Indonesia.
Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 7 Tahun (2014) Tentang Pedoman Uji Toksisitas Nonklinik Secara In Vivo.
de Moura, R.S., A.F. Emiliano, L.C.R. Marins de Carvalho, L.C.R. Souza, D.C. Guedes, T. Tano, A.C. Resende. (2005). Antihypertensive and endothelium-dependent vasodilator effects of Alpinia zerumbet, a medicinal plant. J Car diovasc PharmacolTM, 46, 288–294.
Detrich, H.W., Westerfield, M., Zon, L.I. (2009). Essential zebrafish method : Genetics and genomics. Elseivier. 1st edition. U.K
Eno, A.E., U.E. Ibokette, Ofem, F.B. Uoh, E. Nkanu, N. Azah, J.O. Ibu. (2004). The effects of a negerian species of Viscum album (mistletoe) leaf extraction on the blood pressure of normotensive and DOCA_induced hypertensive rats. Nigerian Journal of Physiological Sci, 19 (1-2), 33-38.
Halliwell, B. and M.C. Gutteridge. (2010). Role of free radicals and catalytic metal ions in human disease. Oxford. Clarendon Press.
Hanien, D.N., Firmansyah, M., Athiroh, N. (2016). Nilai LC50 ikan zebra dewasa yang dipapar oleh dekokta daun benalu teh (Scurulla atropurpurea Bl.Danser) secara akut dan subkronik. Jurnal Bio Komplementer Medicine, 3 (1), 65-70.
Hasri, Y.U., Firmansyah, M., Athiroh, N. (2016). Nilai LCC0 embrio dan larva ikan zebra (Danio rerio) yang dipapar dekokta daun benalu teh (Scurrula atropurpurea Bl. dans.) secara akut. Jurnal Bio Komplementer Medicine, 3 (1), 59-64.
Hikmah, U., Athiroh,, N., Santoso, H. (2017). Kajian sub kronik ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap serum glutamic oxaloacetic transaminase tikus wistar betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2 (2), 30-35.
Jiménez, R., R. López-Sepúlveda , M. Kadmiri , M. Romero , R. Vera , M. Sánchez , F. Vargas , F. O'Valle , A. Zarzuelo , M. Dueñas , C. Santos-Buelga , J. Duarte. (2007). Polyphenols restore endothelial function in DOCA-salt hypertension: role of endothelin-1 and NADPH oxidase. Free Radic Biol Me,. 43(3), 462-73.
Kawamura, E., & Pamudji. (2005). Pharmaceutical composition containing powder or extract of a paraite plant of the loranthaceae family. European Patent Aplication. EP 1 591 126 A2.
Langgheinrich, U. (2003). Bioassay. Vol. 25. 904-912.
Leischke, G.J., & Currie, P.D. (2007). Animal models for human desease: Zebrafish swim into view. Nat Pub Group. 8 : 353-367.
Mahyan, A., Athiroh, N., Santoso, H. (2016). Paparan 28 hari ekstrak metanolik Scurrula atropurpurea terhadap kadar SGPT tikus betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(1), 53-58.
Munawaroh, N.S., Athiroh, N., Santoso, H. (2016). Kajian ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap kadar trigliserida pada tikus wistar betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(1), 59-64.
Murphy, KJ., A.K. Chronopolous, I. Singh, M.A. Francis, H. Moriarty, M.J. Pike, A.H. Turner, N.J. Mann, and J. Sinclair. (2003). Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao) inhibit platelet function. American Journal of Clinical Nutrition, 77 (6), 1466-1473.
Mustofa, B., A. Hayati, dan N. Athiroh. (2013). Peran ekstrak metanolik benalu teh terhadap perbaikan stress oksidatif melalui peningkatan SOD pada tikus hipertensi. Jurnal Ilmiah Bio saintropis, 1 (2), 1-4.
Nugroho, R.T., Herbani, M., Athiroh, N. (2016). Efek dekokta daun benalu teh (Scurrula atropurpurea BL Danser) dosis terapi, MATC, dan LC50 terhadap denyut jantung dan daya tetas embrio ikan zebra (Danio rerio). Jurnal Bio Komplementer Medicine, 3 (1), 51-58.
Ortiz, P.A., and J.L. Garvin. (2001). Intrarenal transport and vasoactive substances in hypertension. Hypertension, 38, 621–624.
Purnamasari, D., Herbani, M., Athiroh, N. (2016). Efek dekokta daun benalu teh (Scurrula atropurpurea Bl. Dans) dosis terapi, MATC, dan Lc50 terhadap luas area yolk sac serta pericardium embrio ikan zebra (Danio rerio). Jurnal Bio Komplementer Medicine, 3 (1), 167-173.
Prastika, N.I., Athiroh, N., Santoso, H. (2017). Pengaruh pemberian sub kronik ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. terhadap kadat kreatinin tikus wistar. e-Jurnal Ilmiah BIOSAINTROPIS, 2(2), 42-48.
Redon, J.M., M.R. Oliva, C. Tormos, V. Giner, J. Chaves, A. Iradi, G.T. Saez. (2003). Antioxidant activities and oxidative sress byproducts in human hypertension. Journal of The American Heart Association, 41, 1096-1101.
Sammad, F.A.H., Athiroh, N., Santoso, H. (2017). Pemberian ekstrak metanolik Scurrula atropurpurea (Bl.) Dans. secara sub kronik terhadap protein total dan albumin tikus betina. e-Jurnal Ilmiah BIOSAINTROPIS, 2(2), 49-54.
Vaziri, N.D., and B. Rodriguez-Iturbe. 2006. Mechanisms of Disease: Oxidative Stress and Inflammation in The Pathogenesis of Hypertension. Nat Clin Pract Nephrol, 282–593.
Wang, S., Kechun Liu, Ximing Wang, Qiuxia He, and Xiqiang Chen. (2010). Toxic effect of celastrol on embryonic development of zebra fish (Danio rerio). Biology Institute of Shandong Academy of Sciences, Jinan 250014, Shandong, China.