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Chitosan, biopolymer, is made of deacetylation of chitin which commonly extracted from Crustacean and can be utilized as an antibacterial agent. This present study aimed to examine the degree of chitosan isolated from the snail shell and determine antibacterial activity against Staphylococcus aureus. Chitosan was isolated through deacetylation of chitin using Knorr methode by adding 60% of NaOH at a ratio of 20:1 (v/b) and refluxed at 100-1400C for 1 hour. It was allowed to be cold then filtered and the solid acquired was neutralized with distilled water. The solids are then dried at 800C for 24 hours. The antibacterial activity against Staphylococcus aureus was carried out on chitosan with various concentration using Kiby Bauer method and statistically analyzed by One Way ANOVA. The result showed that the deacetylation degree of chitosan was 63.09%, and the optimum concentration of chitosan that could inhibit the Staphylococcus aureus bacteria was 500 ppm, with diameter inhibition was 34.33 mm.
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