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Preliminary Detection of Antibacterial Activity of Fishpond Water Bacteria against Aquaculture Pathogenic Bacteria

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Stella Magdalena Sindy Sindy Yogiara Yogiara

Abstract

Aquaculture is currently experiencing massive loss due to the outbreak of pathogenic bacteria. One of the outbreak causes is the development of pathogenic bacterial resistance to the antibacterial. The problem can be solved using microorganisms that can produce new antibacterial compounds. The purpose of this research was to obtain bacteria from fishpond water that could produce antibacterial compounds. About two out of 81 isolates could produce antibacterial compounds. Those two isolates were obtained from saltwater fishponds in North Jakarta (TS2) and Harapan Island (PHY). All fishpond water was grown in marine broth or Luria broth. Extraction of antibacterial compounds was performed using four types of solvents: chloroform, dichloromethane, ethyl acetate, and methanol. Each of the solvents showed a different result. The extraction can only be successfully performed using chloroform and dichloromethane. Extraction using dichloromethane showed a larger inhibitory clear zone than chloroform. Based on 16S rRNA gene sequencing, PHY isolate was identified as Bacillus sp. and TS2 as Acinetobacter sp. In conclusion, isolate TS2 and PHY, which produced antibacterial compounds, showed potential use as aquaculture probiotics.

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Aboshora, W., Lianfu, Z., Dahir, M., Qingran, M., Qingrui, S., Jing, L., Ammar, A. (2014). Effect of extraction Method and Solvent Power on Polyphenol and Flavonoid Levels in Hyphaene Thebaica L Mart (Arecaceae) (Doum) Fruit, and Its Antioxidant and Antibacterial Activities. Tropical Journal of Pharmaceutical Research, 13(12), 2057-2063. https://doi.org/10.4314/tjpr.v13il2.16

Akhter, N., Wu, B., Memon, A. M., & Mohsin, M. (2015). Probiotics and Prebiotics Associated with Aquaculture: A review. Fish & Shellfish Immunology, 45(2), 733-741. https://doi.org/10.1016/j.fsi.2015.05.038

Algammal, A. M., Mabrok, M., Sivaramasamy, E., Youssef, F. M., Atwa, M. H., El-kholy, A. W., Hozzein, W. N. (2020). Emerging MDR-Pseudomonas aeruginosa in Fish Commonly Harbor oprL and toxA Virulence Genes and blaTEM, blaCTX-M, and tetA Antibiotic-Resistance Genes. Scientific Reports, 10(1), 15961. https://doi.org/10.1038/s41598-020-72264-4

Anupama, R., Mukherjee, A., & Babu, S. (2018). Gene-centric Metagenome Analysis Reveals Diversity of Pseudomonas Aeruginosa Biofilm Gene Orthologs in Freshwater Ecosystems. Genomics, 110(2), 89-97. https://doi.org/10.1016/j.ygeno.2017.08.010

Assefa, A., & Abunna, F. (2018). Maintenance of Fish Health in Aquaculture: Review of Epidemiological Approaches for Prevention and Control of Infectious Disease of Fish. Veterinary Medicine International, 2018, 5432497. https://doi.org/10.1155/2018/5432497

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for In Vitro Evaluating Antimicrobial Activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. https://doi.org/10.1016/j.jpha.2015.11.005

Bunnoy, A., Na-Nakorn, U., & Srisapoome, P. (2019). Probiotic Effects of a Novel Strain, Acinetobacter KU011TH, on The Growth Performance, Immune Responses, and Resistance against Aeromonas hydrophila of Bighead Catfish (Clarias macrocephalus Günther, 1864). Microorganisms, 7(12), 613. https://doi.org/10.3390/microorganisms7120613

Carrera, M., Piñeiro, C., & Martinez, I. (2020). Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products. Foods (Basel, Switzerland), 9(8), 1050. https://doi.org/10.3390/foods9081050

Francino, M. P. (2016). Antibiotics and The Human Gut Microbiome: Dysbioses and Accumulation of Resistances. Frontiers in MicrobiologyFrontiers in Microbiology, 6(Article 1543), 1–11. https://doi.org/10.3389/fmicb.2015.01543

Gao, X.-Y., Liu, Y., Miao, L.-L., Li, E.-W., Hou, T.-T., & Liu, Z.-P. (2017). Mechanism of Anti-Vibrio Activity of Marine Probiotic Strain Bacillus pumilus H2, and Characterization of The Active Substance. AMB Express, 7(1), 23-23. https://doi.org/10.1186/s13568-017-0323-3

Karl, P. J., Hatch, A. M., Arcidiacono, S. M., Pearce, S. C., Pantoja-Feliciano, I. G., Doherty, L. A., & Soares, J. W. (2018). Effects of Psychological, Environmental and Physical Stressors on The Gut Microbiota. Frontiers in Microbiology, 9(September), 2013. https://doi.org/10.3389/fmicb.2018.02013

Kuebutornye, F. K. A., Abarike, E. D., & Lu, Y. (2019). A review on The Application of Bacillus as Probiotics in Aquaculture. Fish & Shellfish Immunology, 87, 820-828. https://doi.org/10.1016/j.fsi.2019.02.010

Kumar, M., Jaiswal, S., Sodhi, K. K., Shree, P., Singh, D. K., Agrawal, P. K., & Shukla, P. (2019). Antibiotics Bioremediation: Perspectives on Its Ecotoxicity and Resistance. Environment International, 124(December 2018), 448–461. https://doi.org/10.1016/j.envint.2018.12.065

Li, X., Gao, X., Zhang, S., Jiang, Z., Yang, H., Liu, X., Zhang, X. (2020). Characterization of a Bacillus velezensis with Antibacterial Activity and Inhibitory Effect on Common Aquatic Pathogens. Aquaculture, 523, 735165. https://doi.org/10.1016/j.aquaculture.2020.735165

Liu, Y., De Schryver, P., Van Delsen, B., Maignien, L., Boon, N., Sorgeloos, P., Defoirdt, T. (2010). PHB-Degrading Bacteria Isolated from The Gastrointestinal Tract of Aquatic Animals as Protective Actors Against Luminescent Vibriosis. FEMS Microbiology Ecology, 74(1), 196-204. https://doi.org/10.1111/j.1574-6941.2010.00926.x

Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., & Wade, W. G. (1998). Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Applied and Environmental Microbiology, 64(2), 795-799. https://doi.org/10.1128/aem.64.2.795-799.1998

Olmos, J., Acosta, M., Mendoza, G., & Pitones, V. (2020). Bacillus subtilis, an Ideal Probiotic Bacterium to Shrimp and Fish Aquaculture That Increase Feed Digestibility, Prevent Microbial Diseases and Avoid Water Pollution. Archives of Microbiology, 202(3), 427-435. https://doi.org/10.1007/s00203-019-01757-2

Pérez, M. J., Falqué, E., & Domínguez, H. (2016). Antimicrobial Action Of Compounds from Marine Seaweed. Marine Drugs, 14(3), 52. https://doi.org/10.3390/md14030052

Preetha, R., Jose, S., Prathapan, S., Vijayan, K. K., Jayaprakash, N. S., Philip, R., & Bright Singh, I. S. (2010). An Inhibitory Compound Produced by Pseudomonas with Effectiveness on Vibrio harveyi. Aquaculture Research, 41(10), 1452-1461. https://doi.org/10.1111/j.1365-2109.2009.02436.x

Prem, A. T., Chellaram, C., Kumaran, S., & Shantini, C. F. (2011). Screening for Antibiotic-Producing Marine Bacteria against Fish Pathogens. International Journal of Pharma and Bio Sciences, 2(1), 314-325.

Pundir, R. K., Rana, S., Kaur, A., Kashyap, N., & Jain, P. (2014). Bioprospecting Potential Of Endophytic Bacteria Isolated from Indigenous Plants of Ambala (Haryana, India). International Journal of Pharmaceutical Science and Research, 5(6), 2309-2319. https://doi.org/10.13040/IJPSR.0975-8232.5(6).2309-19

Ren, Q., Yu, M., Li, Y., Zhang, Y., Shi, X., Wu, Y., Zhang, X.-H. (2018). Flavobacterium ovatum sp. nov., a Marine Bacterium Isolated from an Antarctic Intertidal Sandy Beach. International Journal of Systematic and Evolutionary Microbiology, 68(3), 795-800. https://doi.org/10.1099/ijsem.0.002586

Ringø, E., Van Doan, H., Lee, S. H., Soltani, M., Hoseinifar, S. H., Harikrishnan, R., & Song, S. K. (2020). Probiotics, Lactic Acid Bacteria and Bacilli: Interesting Supplementation for Aquaculture. Journal of Applied Microbiology, 129(1), 116-136. https://doi.org/10.1111/jam.14628

Shao, Y., Zhong, H., Mao, X., & Zhang, H. (2020). Biochar-immobilized Sphingomonas sp. and Acinetobacter sp. Isolates to Enhance Nutrient Removal: Potential Application in Crab Aquaculture. Aquaculture Environment Interactions, 12, 251-262.

Shetty, P. R., Buddana, S. K., Tatipamula, V. B., Naga, Y. V. V., & Ahmad, J. (2014). Production of Polypeptide Antibiotic from Streptomyces parvulus and its Antibacterial Activity. Brazilian Journal of Microbiology, 45(1), 303-312. https://doi.org/10.1590/S1517-83822014005000022

Shinn, A. P., Pratoomyot, J., Griffiths, D., Trong, T. Q., Vu, N. T., Jiravanichpaisal, P., & Briggs, M. (2018). Asian Shrimp Production and The Economic Costs of Disease Asian Fisheries Science, 31S, 29-58. https://doi.org/10.33997/j.afs.2018.31.S1.003


Soltani, M., Ghosh, K., Hoseinifar, S. H., Kumar, V., Lymbery, A. J., Roy, S., & Ringø, E. (2019). Genus Bacillus, Promising Probiotics in Aquaculture: Aquatic Animal Origin, Bio-Active Components, Bioremediation and Efficacy in Fish and Shellfish. Reviews in Fisheries Science & Aquaculture, 27(3), 331-379. https://doi.org/10.1080/23308249.2019.1597010

Sujatha, S., & Suresh, A. (2013). Polar and Nonpolar Solvent Extraction and Pharmacological Evaluation of Four Different Parts from Brassica nigra (Koch.) Plant. Journal of Pharmaceutical and Scientific Innovation, 2, 27-29. https://doi.org/10.7897/22774572.02333

Tan, L. T.-H., Lee, L., & Goh, B.-H. (2020). Critical Review of Fermentation and Extraction of Anti-Vibrio Compounds from Streptomyces. Progress in Microbes and Molecular Biology, 3(1), a0000051. https://doi.org/10.36877/pmmb.a0000051.

Thangamani, V., & Rajendran, N. (2016). Total Heterotrophic Bacterial Load in The Gut of Detritus Fishes: A Case Study of Pichavaram Mangrove Environment, Southeast Coast, India. Current World Environment, 11, 778-783. https://doi.org/10.12944/CWE.11.3.12

Tran, N., Rodriguez, U. P., Chan, C. Y., Phillips, M. J., Mohan, C. V., Henriksson, P. J. G., Hall, S. (2017). Indonesian Aquaculture Futures: An Analysis of Fish Supply and Demand in Indonesia to 2030 and Role of Aquaculture Using the AsiaFish Model. Marine Policy, 79(February), 25–32. https://doi.org/10.1016/j.marpol.2017.02.002

Vasiliadou, I. A., Molina, R., Martinez, F., Melero, J. A., Stathopoulou, P. M., & Tsiamis, G. (2018). Toxicity Assessment of Pharmaceutical Compounds on Mixed Culture from Activated Sludge Using Respirometric Technique: The Role of Microbial Community Structure. Science of the Total Environment, 630(15 july 2018), 809–819. https://doi.org/10.1016/j.scitotenv.2018.02.095

Veronica, V., Lay, B. W., & Magdalena, S. (2014). Isolation and Characterization of New Antibiotics from Indonesian Coastal Marine Bacteria. Microbiology Indonesia, 8(3), 1. https://doi.org/10.5454/mi.8.3.1

Xu, B.-H., Ye, Z.-W., Zheng, Q.-W., Wei, T., Lin, J.-F., & Guo, L.-Q. (2018). Isolation and Characterization of Cyclic Lipopeptides with Broad-Spectrum Antimicrobial Activity from Bacillus siamensis JFL15. 3 Biotech, 8(10), 444. doi:10.1007/s13205-018-1443-4. https://doi.org/10.1007/s13205-018-1443-4.

Yi, Y., Zhang, Z., Zhao, F., Liu, H., Yu, L., Zha, J., & Wang, G. (2018). Probiotic Potential of Bacillus velezensis JW: Antimicrobial Activity Against Fish Pathogenic Bacteria and Immune Enhancement Effects on Carassius auratus. Fish & Shellfish Immunology, 78, 322-330. https://doi.org/10.1016/j.fsi.2018.04.055