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Stomata Profile Comparisons in Abaxial and Adaxial Zones of Dendrobium aphyllum and Arachnis flos-aeris Leaves

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Febri Yuda Kurniawan Agung Dwi Santoso


Stomata activity in orchids is related to orchids' adaptation process to their environment. This study aimed to investigate the stomata profile, comprising of anatomical structure and density of the two orchids species, Dendrobium aphyllum, and Arachnis flos-aeris. Data was collected by printing the leaves' surface using glue and observed at 09.00 am and 09.00 pm, then analyzed using Image raster 3.0 software and MS. Excel. The stomata of D. aphyllum and A. flos-aeris were characterized by anomocytic type. Results showed that the highest stomata density was 1177.78 um2, found on the abaxial part of D. aphyllum. The adaxial leaves of both species were classified as low or no stomata. These two orchid species were epiphyte yet grow in different habitats. Arachnis flos-aeris exposed to direct sunlight tends to reduce transpiration rate, resulting in fewer stomata than D. aphyllum less exposed to sunlight.

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Agustini, V., Zebua, L. I., & Wenda, N. (2016). Inventory of native orchids in Makki Sub-District, Lanny Jaya, Papua, Indonesia. Biodiversitas, 17(1), 301–305.

Anosheh, H. P., Moucheshi, A. S., Pakniyat, H., & Pessarakli, M. (2016). Stomatal responses to drought stress. In Parvaiz Ahmad (Ed.), Water Stress and Crop Plants: A Sustainable Approach (Vol. 1, pp. 24–40). John Wiley & Sons, Ltd.

Aono, A. H., Nagai, J. S., Dickel, G. da S. M., Marinho, R. C., de Oliveira, P. E. A. M., & Faria, F. A. (2019). A Stomata Classification and Detection System in Microscope Images of Maize Cultivars. BioRxiv, 55(12), 1–15.

Arif, A., & Ratnawati. (2018). Hubungan kekerabatan anggrek dendrobium berdasarkan karakteristik morfologis dan anatomis daun the relativity relation of dendrobium based on morphological and anatomical leaf characters. Jurnal Prodi Biologi, 7(4), 213–222.

Baillie, A. L., & Fleming, A. J. (2020). The developmental relationship between stomata and mesophyll airspace. New Phytologist, 225(3), 1120–1126.

Bertolino, L. T., Caine, R. S., & Gray, J. E. (2019). Impact of stomatal density and morphology on water-use efficiency in a changing world. Frontiers in Plant Science, 10(March), 225.

Clauw, P., Coppens, F., Beuf, K. De, Dhondt, S., Daele, T. Van, Maleux, K., … Systems, P. (2015). Leaf Responses to Mild Drought Stress in Natural Variants of Arabidopsis. Plant Physiology, 167(March 2015), 800–816.

Darmawati, I. A. P., Rai, I. N., Dwiyani, R., & Astarini, I. D. A. A. Y. U. (2018). Short Communication : The diversity of wild Dendrobium ( Orchidaceae ) in Central Bali , Indonesia. Biodiversitas, 19(3), 1110–1116.

Dharma, K. S., & Maryani. (2018). The effect of priming duration with salicylic acid under salinity stress on growth and leaf anatomy of sweet corn ( Zea mays L .). Ilmu Pertanian (Agricultural Science), 3(1), 36–45.

Fan, J., He, R., Zhang, Y., & Jin, X. (2014). Systematic significance of leaf epidermal features in Holcoglossum (Orchidaceae). PLoS ONE, 9(7), 101557.

Fardhani, I., Kisanuki, H., & Parikesit. (2015). Diversity of Orchid Species in Mount. In Proceedings of the 22nd Tri-University International Joint Seminar and Symposium 2015 (pp. 1–4).

Garvita, R. V., & Wawangningrum, H. (2020). Stomata cells studies of Paraphalaenopsis spp. From in vitro and greenhouse condition. Biodiversitas, 21(3), 1116–1121.

Harrison, E. L., Arce Cubas, L., Gray, J. E., & Hepworth, C. (2020). The influence of stomatal morphology and distribution on photosynthetic gas exchange. Plant Journal, 101(4), 768–779.

Haryanti, S. (2010). Jumlah dan Distribusi Stomata pada Daun Beberapa Spesies Tanaman Dikotil dan Monokotil. Anatomi Fisiologi, XVIII(2), 21–28.

Hong, T., Lin, H., & He, D. (2018). Characteristics and correlations of leaf stomata in different Aleurites Montana provenances. PLoS ONE, 13(12), 1–10.

Lee, J. S. (2010). Stomatal Opening Mechanism of CAM Plants. J. Plant Biol, 53(February 2010), 19–23.

Males, J., & Grif, H. (2017). Stomatal Biology of CAM Plants. Plant Physiology, 174(June), 550–560.

Matthews, J. S. A., & Lawson, T. (2019). Climate Change and Stomatal Physiology. (J. Roberts, Ed.), Annual Plant Reviews online (Vol. 2). United Kingdom: Jhon Wiley & Sons, Ltd.

Nunes, T. D. G., Zhang, D., & Raissig, M. T. (2020). Form, development and function of grass stomata. Plant Journal, 101(4), 780–799.

Puspitaningtyas, D. M. (2018). Orchid exploration in Mount Bintan Besar protected forest, bintan Island, Riau Islands Province, Sumatra, Indonesia. Biodiversitas, 19(3), 1081–1088.

Raissig, M. T., Matos, J. L., Gil, M. X. A., Kornfeld, A., Bettadapur, A., Abrash, E., … Bergmann, D. C. (2017). Mobile MUTE specifies subsidiary cells to build physiologically improved grass stomata. Science, 355(6330), 1215–1218.

Rindyastuti, R., & Hapsari, L. (2017). Adaptasi Ekofisiologi Terhadap Iklim Tropis Kering : Studi Anatomi Daun Sepuluh Jenis Tumbuhan Berkayu ( Ecophysiological adaptation to dry tropical climate : a study of foliar anatomic structure of ten woody plant species). Jurnal Biologi Indonesia, 13(1), 1–14.

Rindyastuti, R., Nurfadilah, S., Rahadiantoro, A., Hapsari, L., & Abiwijaya, I. K. (2018). Leaf Anatomical Characters of Four Epiphytic Orchids of Sempu Island, East Java, Indonesia: The Importance in Identification and Ecological Adaptation. Biodiversitas, 19(5), 1902–1905.

Rompas, Y. (2011). Struktur Sel Epidermis dan Stomata Daun Beberapa Tumbuhan Suku Orchidaceae. Jurnal Bios Logos, 1(1), 13–19.

Rudall, P. J., Chen, E. D., & Cullen, E. (2017). Evolution and development of monocot stomata. American Journal of Botany, 104(8), 1122–1141.

Santelia, D., & Lawson, T. (2016). Rethinking guard cell metabolism. Plant Physiology, 172(3), 1371–1392.

Sma-Air, S., & Ritchie, R. J. (2020). Photosynthesis in a Vanda sp orchid with Photosynthetic Roots. Journal of Plant Physiology, 251(Agustus 2020), 153187.

Soares, A. S., Driscoll, S. P., Olmos, E., Harbinson, J., Arrabaça, M. C., & Foyer, C. H. (2008). Adaxial/abaxial specification in the regulation of photosynthesis and stomatal opening with respect to light orientation and growth with CO 2 enrichment in the C4 species Paspalum dilatatum. New Phytologist, 177(1), 186–198.

Ullah, F., Ayaz, A., Saqib, S., Parmar, G., Bahadur, S., & Zaman, W. (2020). Taxonomic implication of leaf epidermal anatomy of selected taxa of Scrophulariaceae from Pakistan. Microscopy Research and Technique, 83(12), 1–10.

Zhang, S., Yang, Y., Li, J., Qin, J., Zhang, W., Huang, W., & Hu, H. (2018). Physiological diversity of orchids. Plant Diversity, 40(4), 196–208.

Zhang, Z. S., Li, Y. T., Gao, H. Y., Yang, C., & Meng, Q. W. (2016). Characterization of photosynthetic gas exchange in leaves under simulated adaxial and abaxial surfaces alternant irradiation. Scientific Reports, 6(January), 1–11.

Zwieniecki, M. A., Haaning, K. S., Boyce, C. K., & Jensen, K. H. (2016). Stomata design principles in synthetic and real leaves. Journal of the Royal Society Interface, 13(124), 20160535.